Chronic ingestion of alcohol modulates expression of ...
Cells were then stained with fluorescence labeled antibodies against cell markers of alveolar macrophage, eosinophil, neutrophil and lymphocyte (1:100) for 30 min on ice. After washing, cells were analyzed by a FACSarray bioanalyzer (BD Bioscience, San Jose, CA, USA). Data were analyzed by the software FlowJo. Extraction of nuclear proteins
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